(77) Hust, M., Frenzel, A., Schirrmann, T. and Dübel, S. (2014). Michael Hust (* 1972 in Oldenburg (Oldb)) ist ein deutscher Biologe und Professor für Biotechnologie an der Technischen Universität Braunschweig. In: Antibody engineering Vol 1, Ed: Kontermann, R. and Dübel, S.. (43) Hust, M. and Mersmann, M. (2010). and Kontermann, R.E. Antibody Affinity and Stability Maturation by Error-Prone PCR. (2018). ➜ Clusters of Excellence: In the Excellence Strategy of the Federal Government and States in 2018, TU Braunschweig was successful with two applications. (10) Hust, M., Toleikis, L. and Dübel, S. (2005). Dübel, S. and Reichert, J.M.. (83) Wilke, S. and Hust, M. (2014). and Rhiel, E. (2003). 1 Job ist im Profil von Wolfgang Niemeier aufgelistet. (2016). and von Nickisch-Rosenegk, M. (2016). Picture credits: Michael Hust/TU Braunschweig. (133) Kopertekh, L., Meyer, T., Freyer, C. and Hust M. (2019). (78) Hülseweh, B., Rülker, T., Pelat, T., Langermann, C., Frenzel, A., Schirrmann, T., Dübel, S., Thullier, P. and Hust, M. (2014). (81) Fuchs, M., Kämpfer, S., Helmsing, S., Spallek, R., Oehlmann, W., Prilop, W., Frank, R., Dübel, S., Mahavir, S. and Hust, M. (2014). (18) Schirrmann, T., Hust, M., Konthur, Z., Frank, R., Toleikis, L. and Dübel, S. (2007). Affinity-matured variants derived from nimotuzumab keep the original fine specificity and exhibit superior biological activity. It works great because we can rely on each other,” says Nora Langreder describing the collaboration. Die Antikörperfabrik: Antikörper für jedes Protein. Technische Universität Braunschweig Email: s.duebel@tu-braunschweig.de (102) Kuhn, P., Fühner, V., Moreira, G.M.S.G., Frenzel, A., Miethe, S. and Hust, M. (2016). “This is a method of producing antibodies in the test tube without the immunisation of animals,” explains Stefan Dübel, one of the inventors of the method. (95) Tomszak, F., Weber, S., Zantow, J., Schirrmann, T., Hust, M. and Frenzel, A. In cooperation with the biotechnology company YUMAB, a spin-off of the TU Braunschweig, the Carolo-Wilhelmina researchers have investigated several thousand antibodies. September 2019 die Norden Vaccines GmbH gegründet. A roadmap to generate renewable protein binders to the human proteome. seroreactive peptides using ORFeome Phage Display. Efficient production of soluble recombinant single chain Fv fragments by a Pseudomonas putida strain KT2440 cell factory. 12/2014 Appointed as professor for biotechnology (außerplanmäßiger Professor). (AA), Dipl.-Ing. Utilisation of antibody microarrays for the selection of specific and informative antibodies from recombinant library binders of unknown quality. (105) Miethe, S., Mazuet, C., Liu, Y., Tierney, R., Rasetti-Escargueil, C., Avril, A., Frenzel, A., Thullier, P., Pelat, T., Urbain, R., Fontayne, A., Sesardic, D., Hust, M. and Popoff, M.-R. (2016). It's current labs are located in the Biocentre and the BRICS. Isolation of a human-like antibody fragment (scFv) that neutralizes ricin biological activity. Expertenpools des Nationalen Ausschuss für den Schutz von für wissenschaftliche Zwecke verwendeten Tieren, Scientific Coordinator of the EC consortium, Member of Verband Biologie, Biowissenschaften und Biomedizin in Deutschland (. Erfahren Sie mehr über die Kontakte von Wolfgang Niemeier und über Jobs bei ähnlichen Unternehmen. (2011). Braunschweiger Forscher gehen den Zecken an den Kragen Jüngste Ausgründung aus der TU Braunschweig Wissenschaftler aus Braunschweig haben heute, am 11. A SARS-CoV-2 neutralizing antibody selected from COVID-19 patients by phage display is binding to the ACE2-RBD interface and is tolerant to known RBD mutations. Delivery of antibodies to the cytosol - debunking the myths. Improving phage display throughput by using Hyperphage, miniaturized titration and pVIII (g8p) ELISA. (127) Froude, J.W., Herbert, A.S., Pelat, T., Miethe, S., Zak, S.E., Brannan, J.M., Bakken, R.R., Steiner, A.R., Yin, G., Hallam, T.J., Sato, A.K., Hust, M., Thullier, P. and Dye, J.M. (2016). (99) Rohrbeck, A., Fühner, V., Schröder, A., Hagemann, S., Vu, X.-H., Berndt, S., Hust, M., Pich, A. and Just, I. A One-Step Process for the Construction of Phage Display scFv and VHH Libraries. In the interview they told us about the search for antibodies at Carolo-Wilhelmina, what antibody libraries are and why teamwork is so important for their research. Kai-Thomas Schneider adds: “We have all learned a lot and have grown together as a team. (21) Jordan, E., Al-Halabi, L., Schirrmann, T., Hust, M. and Dübel, S. (2007). (122) Moreira, G.M.S.G., Fühner, V. and Hust, M. (2018). (2014). Selection of recombinant human antibodies. antibodies from a single cell clone of human blood, are attached to the surface of a phage, a kind of ‘transport virus’. Production of single chain Fab (scFab) in Bacillus megaterium. Die Grundlage für ihre Arbeit sind die neutralisierenden Antikörper gegen Covid-19, die die Technische Universität Braunschweig gemeinsam mit dem Biotechnologieunternehmen Yumab GmbH im Corat Konsortium entwickelt hat. In: Antibody engineering Vol 2, Ed: Kontermann, R. and Dübel, S.. (44) Breitling, F., Broders, O., Helmsing, S., Hust, M. and Dübel, S. (2010). Passive immunotherapy, which is also being researched by the CORAT consortium, could help here: the administered antibodies could block the pathogen and thus provide protection until the immune system has produced its own antibodies. Heterologe Expression von rekombinanten Proteinpharmazeutika. (103) Frenzel, A., Schirrmann, T. and Hust, M. (2016). A human scFv antibody generation pipeline for proteome research. Affinity maturation by phage display. (123) Dübel, S., Hust, M., Kügler, J., Kuhn, P., Frenzel, A., Krah, S. and Zielonka, S. (2018). (63) Rülker, T., Voß, L., Thullier, P., O'Brien, L.M., Pelat, T., Perkins, S.D., Langemann, C., Schirrmann, T., Dübel, S., Marschall, H.-J., Hust, M. and Hülseweh, B. The work now feels like a doctoral thesis on a much larger scale and in less time, which we are all doing together as a team. (2018). Phage display, Ed: Lim, T.S. (20) Hust, M., Dübel, S. and Schirrmann, T. (2007). “Finding such neutralizing antibody candidates was our most important milestone on the way to a passive immunotherapy against Covid-19, but there are still many more steps and tests to be done before the final drug is ready for use,” says Michael Hust. Herzstiftung fördert Entwicklung neuer Antikörper-Therapie gegen Covid-19. (84) Hust, M., Frenzel, A., Tomszak, F., Kügler, J. and Dübel, S. (2014). Michael Hust weiß, wovon er redet, wenn er über die Borreliose spricht. (131) Fühner, V., Heine, P.A., Zilkens, K., Meier, D., Roth, K.D.R., Moreira, G.M.S.G., Hust, M. and Russo, G. (2019). (34) Pelat, T., Hust, M., Hale, M., Lefranc, M.-P., Dübel, S. and Thullier, P. (2009). 5 Technische Universität Braunschweig, Institute for Biochemistry, Biotechnology and Bioinformatics, Department of Biotechnology, Braunschweig, Germany. High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells. ETH Zürich), M.Arch. Generating recombinant antibodies for research, diagnostics and therapy using phage display. (22) Schirrmann, T., Hust, M. and Dübel, S. (2007). (107) Zantow, J., Just, S., Lagkouvardos, I., Kisling, S., Dübel, S., Lepage, P., Clavel, T. and Hust, M. (2016). (132) Ramli, R.S., Moreira, G.M.S.G., Zantow, J., Goris, M.G.A., Nguyen, V.K., Novoselova, N., Pessler, F. and Hust, M. (2019). Die TU Braunschweig (Prof. Michael Hust) ist Teil des COVID19-Konsortiums "ATAC" im Rahmen des EU Horizon2020 Corona-Förderprogramms. Curriculum vitae 10/1993 - 7/1999 Studies of Biology at the Carl-von-Ossietzky Universität, Oldenburg. Vertical migration behaviour of diatom assemblages of Wadden Sea sediments (Dangast/Germany): A study using Cryo-scanning electron microscopy. Oligomeric forms of single chain immunoglobulin (scIgG). (2017) The European AntibotABE Framework Program and Its Update: Development of Innovative Botulinum Antibodies. The influence of antibody fragment format on phage display based affinity maturation of IgG. Parameters affecting the display of antibodies on phage. The invasin D protein from Yersinia pseudotuberculosis selectively binds the Fab region of host antibodies and affects colonization of the intestine. Email: m.hust@tu-braunschweig.de Expression rekombinanter Proteinpharmazeutika. Aufbauend auf den Forschungsergebnissen des Konsortiums hat das Biotechnologieunternehmen YUMAB, eine Ausgründung der TU Braunschweig, die CORAT Therapeutics GmbH gegründet. Pyruvate dehydrogenase complex - enzyme 2, a new target for. Discovery of Leptospira spp. (35) Schütte, M., Thullier, P., Pelat, T., Wezler, X., Rosenstock, P., Hinz, D., Kirsch, M.I., Hasenberg, M., Frank, R., Schirrmann, T., Gunzer, M., Hust, M. and Dübel, S. (2009). “At the institute we have numerous of these antibody gene libraries. Initiiert wurde das CORAT Konsortium von Professor Stefan Dübel und Professor Michael Hust vom Institut für Biochemie, Biotechnologie und Bioinformatik der TU Braunschweig. Development of recombinant antibodies for biodefense using phage display. (136) Tenspolde, M., Zimmermann, K., Weber, L.C., Hapke, M., Lieber, M., Dywicki, J., Frenzel, A., Hust, M., Galla, M., Buitrago-Molina, L.E., Manns, M.P., Jaeckel, E. and Hardtke-Wolenski, M. (2019). (104) Mendonça, M., Moreira, G.M., Conceição, F.R., Hust, M., Mendonça, K.S., Moreira, Â.N., França, R.C., da Silva, W.P., Bhunia, A.K. 38106 Braunschweig An immunochemical in situ approach to detect adaptation processes in the photosynthetic apparatus of diatoms of the Wadden Sea sediment surface layers. (12) Konthur, Z., Hust, M. and Dübel, S. (2005). Michael Hust studierte von 1993 bis 1999 Biologie an der Carl von Ossietzky Universität Oldenburg . Originally known as Collegium Carolinum, it was established in 1745 as a mid-way institution between high school and university, teaching technical and mathematical subjects as well as fine arts and humanities. Detection and Quantification of ADP-Ribosylated RhoA/B by Monoclonal Antibody. (50) Bourbeillon, J., Orchard, S., Benhar, I., Borrebaeck, C., de Daruvar, A., Dübel, S., Frank, R., Gibson, F., Gloriam, D., Haslam, N., Hiltker, T., Humphrey-Smith, I., Hust, M., Juncker, D., Koegl, M., Konthur, Z., Korn, B., Krobitsch, S., Muyldermans, S., Nygren, P.-A., Palcy, S., Polic, B., Rodriguez, H., Sawyer, A., Schlapshy, M., Snyder, M.,Stoevesandt, O., Taussig, M.J., Templin, M., Uhlen, M., van der Maarel, S., Wingren, C., Hermjakob, H. and Sherman, D. (2010). There, SARS-CoV-2 viruses are used to test whether the antibodies can block these viruses. (134) Chauvin, D., Hust, M., Schütte, M., Chesnay, A., Parent, C., Moreira, G.M.S.G, Arroyo, J., Sanz, A.B., Pugnière, M., Martineau, P., Chandenier, J., Heuzé-Vourc’h, N.  and Desoubeaux, G. (2019). (143) Moreira, G. M.S, Köllner, S.M.S., Helmsing, S., Jänsch, L., Meier, A., Gronow, S.,  Boedeker, C.,  Dübel, S,  Mendonça, M.,  Moreira, A. N., Conceição, F. R., and Hust, M. (in press). Single chain antibodies as tools to study TGF-β regulated SMAD proteins in proximity ligation-based pharmacological screens. In: Encephalitis / Book 3, Ed: Hayasaka, D.. (58) Chahboun, S., Hust, M., Liu, Y., Pelat, T., Miethe, S., Helmsing, S., Jones ,R.G., Sesardic, D. and Thullier, P. (2011). (129) Chung, S.Y., Schöttelndreier, D., Tatge, H., Fühner, V., Hust, M., Beer, L.A. and Gerhard, R. (2018). Encapsulation of Proteins in Hydrogel Carrier Systems for Controlled Drug Delivery: Influence of Network Structure and Drug Size on Release Rate. Institute of Biochemistry, Biotechnology and Bioinformatics (13) Hust, M., Meysing, M, Schirrmann, T., Selke, M., Meens, J., Gerlach, G.-F. and Dübel, S. (2006). (109) Noyan, F., Zimmermann, K., Hardtke-Wolenski, M., Knoefel, A., Schulde, E., Geffers, R., Hust, M., Huehn, J., Galla, M., Morgan, M., Jokuszies, A., Manns, M.P., Jaeckel, E. (2017). and Hust, M.. (116) Frenzel, A., Kügler, J., Helmsing, S., Meier, D., Schirrmann, T., Hust, M. and Dübel, S. (2017). The antibody gene libraries and the antibodies for the tests are generated by the PhD students together with the rest of the team led by Professor Stefan Dübel and Professor Michael Hust from the Institute of Biochemistry, Biotechnology and Bioinformatics using the antibody phage display. When we are ill, our immune system forms antibodies against the pathogens (antigens). “These blueprints show the typical sequence of amino acids that determine how particular antibodies look. Cell-free synthesis of functional antibodies using a coupled in vitro transcription-translation system based on CHO cell lysates. (47) Naseem, S., Meens, J., Jores, J., Heller, M., Dübel, S., Hust, M. and Gerlach, G.F. (2010). Identification of immunogenic proteins and generation of antibodies against Salmonella Typhimurium using phage display. (2021). 5/2011 Habilitation (professorial dissertation, venia legendi for biotechnology), appointed as Privatdozent (PD). (75) Steinwand, M., Droste, P., Frenzel A., Hust, M., Dübel, S. and Schirrmann, T. (2014). (68) Bertz, A., Wöhl-Bruhn, S., Miethe, S., Tiersch, B., Koetz, J., Hust, M., Bunjes, H. and Menzel, H. (2013). Identification of a putative Crf splice variant and generation of recombinant antibodies for the specific detection of Aspergillus fumigatus. Bildnachweis: Michael Hust/TU Braunschweig. and Hust, M.. (121) Zantow, J., Moreira, G.M.S.G., Dübel, S. and Hust, M. (2018. (16) Jordan, E., Hust, M., Roth, A., Biedendieck, R., Schirrmann, T., Jahn, D. and Dübel, S. (2007). since 12/2002 Group leader at the Institut für Biochemie und Biotechnologie, Abteilung Biotechnologie, Technische Universität Braunschweig headed by Prof. Dr. Stefan Dübel. BIOSpektrum (Springer-Verlag, Heidelberg). (108) Postel, S., Deredge, D., Bonsor, D.A., Yu, X., Diederichs, K., Helmsing, S., Vromen, A., Friedler, A., Hust, M., Egelman, E.H., Beckett, D., Wintrode, P.L. C., Jordan, E., El-Ghezal, A., Schirrmann, T., Hust, M. und Jostock, T. (2005). (70) Zhou, M., Meyer, T., Koch, S., Koch, J., Brill, B., von Briesen, H., Benito, J.M., Soriano, V., Haberl, A., Bickel, M., Dübel, S., Hust, M. and Dietrich, U. Phage display, Ed: Lim, T.S. (89) Droste, P., Frenzel, A., Steinwand, M., Pelat, T., Thullier, P., Hust, M., Lashuel, H. and Dübel, S. (2015). (124) Russo, G., Theisen, U., Fahr, W., Helmsing, S., Hust, M., Köster R.W. BDA Braunschweig geht zurück an die Uni - Am Institut für Tragwerksentwurf (ITE) der TU Braunschweig wird unter der Leitung von Univ. Using the antibody gene libraries, the team is now testing which antibodies bind to the protein envelope of the corona virus and thus prevent it from intruding into other cells. and Reinard, T. (2002). A total of 20 µg CMX VHH-Fc plasmid DNA and 60 µL PEI (1 mg/mL) were used to transiently transfect 5 × 10 6 HEK293T cells. (28) Kirsch, M.I., Hülseweh, B., Nacke, C., Rülker, T., Schirrmann, T., Marschall, H.-J., Hust, M. and Dübel, S. (2008). (98) Blokzijl, A., Zieba, A., Hust, M., Schirrmann, T., Helmsing, S., Grannas, K., Hertz, E., Moren, A., Chen, L., Soderberg, O., Moustakas, A., Dübel, S. and Landegren, U. Regulatory T cells engineered with a novel insulin-specific chimeric antigen receptor as a candidate immunotherapy for type 1 diabetes. (2017). (5)Department of Biochemistry and Biotechnology, Technical University Braunschweig, 38106 Braunschweig, Germany. The CORAT consortium was initiated by Professor Stefan Dübel and Professor Michael Hust from the Institute for Biochemistry, Biotechnology and Bioinformatics at the TU Braunschweig together with Professor Gundram Jung from the University of Tübingen. (7) Schirrmann, T., Hust, M. und Dübel, S. (2005). Sehen Sie sich auf LinkedIn das vollständige Profil an. International Microbiology 2, 103-110, Stellen der TU Braunschweig Jobbörse des Career Service Merchandising Sponsoring- & Spendenleistungen Drittmittelgeförderte Forschungsprojekte, Semestertermine Lehrveranstaltungen Studiengänge von A-Z Informationen für Erstsemester TUCard, Status GITZ-Dienste Handbuch für TYPO3 (Intern) Corporate Design-Toolbox (Intern) Glossar (DE-EN) Meine Daten ändern Hochschulöffentliche Bekanntmachungen, Technische Universität Braunschweig Universitätsplatz 2 38106 Braunschweig Postfach: 38092 Braunschweig Telefon: +49 (0) 531 391-0. PMID: 31953428 PMCID: PMC6969050 DOI: 10.1038/s41598-019-57103-5 Abstract Diphtheria is an infectious disease caused by Corynebacterium diphtheriae. Structural differences of amyloid-β fibrils revealed by antibodies from phage display. (56) Lüders, S., David, F., Steinwand, M., Jordan, E., Hust, M., Dübel, S. and Franco-Lara, E. (2011). An der TU Braunschweig werden – im Gegensatz zu den bisher meist in Tieren erzeugten Antiseren – rein menschliche Antikörper mithilfe der Antikörperphagendisplay-Technologie entwickelt. To support the CORAT consortium they have temporarily put their own projects on hold. They enjoy working in the consortium, and it shows: “We are happy to be able to make a contribution to helping in the current situation,” says Nora Langreder, and Kai-Thomas Schneider adds: “This includes working on weekends or holidays. Werden Sie Mitglied unserer akademischen Gemeinschaft - bewerben Sie sich an der TU Braunschweig! Trends in Biotechnology 22, 8-14, (4) Meyer, T., Hust, M., Marquardt, J., Krumbein, W.E. : 0531 391-5760 Inhibition of HER3 activation and tumor growth with a human antibody binding to a conserved epitope formed by domain III and IV. and Hust, M.. (118) Avril, A., Miethe, S., Hust, M. and Pelat, T. (2018). (110) Frenzel, A., Schirmann, T., Dübel, S. and Hust, M. (2017). Human Monoclonal Antibodies: Methods and Protocols, Ed: Steinitz, M.. (73) Kügler, J., Zantow, J., Meyer, T., and Hust, M. (2013). The binary toxin CDT of Clostridium difficile as tool for intracellular delivery of bacterial glucosyltransferase domains. & Schirrmann, T. (2020) Rekombinante, vollständig menschliche Antikörper zur Behandlung akuter COVID-19. Minimum Information about a Protein Affinity Reagent (MIAPAR). With this information, we can produce them again and again.”, This small tube contains an antibody library with about 10 billion antibody blueprints. Development of human antibody fragments using antibody phage display for the detection and diagnosis of Venezuelan equine encephalitis virus (VEEV). Single chain (scFab) fragment. (140) Sellmann, C., Pekar, L., Bauer, C., Ciesielski, E., Krah, S., Becker, S., Toleikis, L., Kügler, J., Frenzel, A., Valldorf,. P., Sesardic, D. and Hust, M. (2015). Vor gut zwei Jahren war das, im Dänemark-Urlaub. (6)Institute of Toxicology, Hannover Medical School, 30625 Hannover, Germany. Antibody Phage Display: Antibody Selection in Solution Using Biotinylated Antigens. Construction of macaque immune libraries. (100) Schieferdecker, A., Oberle, A., Thiele, B., Hofmann, F., Göthel, M, Miethe, S., Hust, M., Braig, F., Voigt, M., Koch-Nolte, F., Haag, F., Alawi, M., Indenbirken, D., Grundhoff, A., Bokemeyer, C., Bacher, U., Kröger, N. and Binder, M. (2016). (2018). (96) Kibat, J., Schirrmann, T., Knape, M.J., Helmsing, S., Meier, D., Hust, M., Schröder, C., Bertinetti, D., Pardes, K., Funk, M., Lages, A., Giese, N., Herberg, F. W., Dübel, S. and Hoheisel, J.D. Generation of recombinant antibodies against toxins and viruses by phage display for Diagnostics and Therapy. (139) Wenzel, E.V., Bosnak, M., Tierney, R., Schubert, M., Brown, J., Dübel, S., Efstratiou, A., Sesardic, D., Sticking, P. and Hust, M. (2020). Braunschweig. and Dübel, S. (2018). Production of Single Chain Fragment Variable (scFv) Antibodies in Escherichia coli Using the LEX Bioreactor. “The next step will be clinical trials, which hopefully can start in autumn,” says Stefan Dübel. (37) Thie, H., Binius, S., Schirrmann, T., Hust, M. and Dübel, S. (2009). Generation and characterization of protective antibodies to Marburg virus. Massensensitives Sensor-Fließsystem zur CRP-Diagnostik.